Generation of induced pluripotent stem cell lines from adult rat cells.

Supplemental Experimental Procedures Cell culture. Rat bone marrow cells (BMC) were cultured in αDMEM culture medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum. Rat primary ear fibroblasts (PEF) were cultured in DMEM culture medium (Invitrogen) supplemented with 10% fetal bovine serum. Rat iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) in Knockout DMEM supplemented with 10% ES cell qualified fetal bovine serum, 10% KnockOut serum replacer, 0.1 mM non-essential amino acids, 1 mM L-glutamine, and 0.1 mM ß-mercaptoethanol (ES media) (all from Invitrogen, Carlsbad, CA). Rat iPS cells were trypsinized into single cells with TTL trypsin and split at a ratio of 1:10 every 3 days. To form embryoid bodies, rat iPS cells were trypsinized into single cells and transferred to a Petri dish in differentiation medium consisting of DMEM (Invitrogen) supplemented with 10% fetal bovine serum.